If you could go back in time and do, or bring, one thing to mess up the timeline what would it be?
You're viewing a single thread.
Ending the first living cell on earth. If we are alone in the universe, preventing life from forming would do the most to change the timeline.
By doing that you would leave behind so many new and much more complex cells (modern bacteria, yeas, maybe even spores) You would probably boost evolution by millions of years!