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Tape over the mouse laser is always a good one
On the computer thread, changing the keys on their keyboard is also fun. Works better if they are a touch typist as well, as they may go several hours/days before noticing, until they have to look for that one special key. :)
Numpad * and / are good for that, I touch type but always have to look at those.
If we're talking computers, screenshot their desktop, set it as background, move taskbar to top, auto hide it, right-click desktop and choose hide icons.
Advanced mode: also set screen flip to 180 degrees and flip the background so it looks right side up.
I did the screen flip thing to a coworker once. And then he just took the monitor and turned it upside down. Didn't see that coming.
Plug in a wireless keyboard/mouse and do things, like move the mouse, or write "chicken" every so often?
I got out of a presentation once in high school by doing that. Put a USB keyboard dongle in the computer, and kept randomly entering characters whenever someone tried to log into their Google account to get a presentation, and alt+f4 whenever someone opened programs
I added this to another similar comment:
Follow it up by placing a tiny bit of clear soft plastic in with the laser. It doesn't stop the mouse from working, but if you were you draw a straight line the cursor wobbles slightly.
You people are monsters