Groups of more than 25 and loudspeakers are no longer allowed to reduce the impact of mass tourism.
New rules banning loudspeakers and limiting tour group sizes to 25 people have come into force in Venice.
The measures have been introduced to limit the impact of over-tourism on the Italian city, officials say.
The canals of Venice's historic quarter make the city one of the most visited places in Europe.
Venice introduced a €5 (£4) daily entry fee earlier this year, after initially banning cruise ships from docking in the quarter in 2021.
Over-tourism is widely regarded as one of the most urgent issues for Venice, which has a population of around 250,000 people and saw more than 13 million visitors in 2019.
You can still make quality of life improvements for the residents while also handling climate change. It's a myth that a local government can only one thing at a time.
It's up there... somewhere in the top ten at least, just gotta hammer out a few other pressing issues, like finding that guy who keeps sticking his gum under the benches.