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Study: Sanitation Effectiveness of 3D-printed Parts for Food and Medical Applications

> Abstract Sanitation effectiveness of 3D-printed parts for food and medical applications has been established in a 4-month lab study and controlled tests. The present study examined the continued use of sanitation techniques across 3 more months of testing and experimentation in household kitchens. Multiple specimens of the most common thermoplastics for Fused Filament Fabrication (FFF), were printed with a range of settings to test for pathogen contamination, biofilm production, bacteria, or other pathogens masking (hiding) in the layer lines, gaps, and other imperfections of said prints. This study investigates methods of sanitation and cleaning to reduce or eliminate pathogens along with its biofilms from the defects and interstitial spaces that naturally occur in FFF printing. Results from various testing methods used in hospitals and FDA approved microbial surface testing, indicate that 3D printed parts of PLA/PLA+ (Polylactic Acid), and PETG (Polyethylene terephthalate glycol) can be cleaned to safe levels using warm water (120 °F), and non-concentrated dish soap. Examination and verification of cleanliness were completed via Petri dish preparations, and protein residue testing. It was found that Colony Forming Units (CFU) and Plaque Forming Units (PFU) had been reduced by 90%. Experimental results indicate that using 2g of baking soda, when used with soapy water, eliminates biofilms by chemical and physical action, neutralizes acidic bacteria, and removes mucus. It is recommended and tested by surgical technicians, that a 2-minute room temperature bleach water soak (200ppm), after washing and rinsing should be done to ensure pathogens are at safe levels. Acetic acid from vinegar was tested as well via petri dish for CFU reduction and can effectively eradicate biofilms due to the ability to penetrate the biofilm matrix and the cell membrane. It is noted to the reader that sanitation in this context refers to the method of bringing a surface or object to safe levels of cleanliness for food or medical preparation and storage. Furthermore, mass spectrometry readings indicate that no contamination from heavy metals, or other toxins are present in PLA+, and PETG before and after printing. Lastly, filaments made from a pull-trusion method from recycled soda or water bottles has been tested as well. When using 3D-printed items for liquids, it is highly recommended to coat the 3D-printed parts in resin. This is a simple, fast, safe, and very effective way to smooth parts to ensure easy cleaning.

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Patlabor Day 2023 (by Aurora)

cross-posted from: https://sopuli.xyz/post/13807898

> Artist: Aurora | pixiv > > Full quality: .jpg 6 MB (3100 × 2920)

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Autobahn Jager 90 by PatsoCatso

https://x.com/PatsoCatso/status/1804644448657133895

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Samus Aran from Metroid by Hungry Clicker

https://x.com/click_burgundy/status/1804974500100120637

Edit - fixed title. Samus Aran not Arab.

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New York’s Fat Beach Day gives plus-size people a space to be themselves
  • Thanks.

    I do eat fruit. Freaking love it. At least 1 apple a day, they are my fav, and often more. I try to eat veggies as much as possible and skip meat when possible. Fiber hasn't been a huge focus for me, but it's worth a shot. I've got a refried beans recipe I freaking love. I wish my kids liked it. My veggie chili is also great, but, you know, kids.

  • New York’s Fat Beach Day gives plus-size people a space to be themselves
  • They need cooking classes, and education around how to properly estimate calories.

    Nope. I count every calorie. I'm shooting for 2300 but struggle to hit that. I usually end up at 2600 or more. I cook 80% of my own food. I bake my own bread. I make my own snacks. I know exactly why I'm fat. I can't stop being hungry. I feel full around 800-900 calories, no matter what I'm eating. (pizza is an exception, because I feel full around 1200 calories, so I avoid it.)

    Imagine walking, chest deep, against a slow moving river, every second of the day. You can push against it and it works, but it's hard. One slip up and you're floating backwards. You know how to make progress, but it's takes a shit load of effort and one mistake and you just. Fucking. Can't. Today.

    Add that into everything else wrong with my life. I only have energy for so many things. I have to triage. Kids, wife, bills, personal happiness, other responsibilities. Can't do them all.

    Trust me, I hate myself with every bite, but it's the only way to shut up that hungry voice.

  • OrcaSlicer V2.1.0 Official Release
  • Can someone give me a brief intro to orca slicer? Who is it made by and what's it's quirk?

    For example prusa slicer, made by prusa, prioritizes user interface and has powerful almost modeling features (text, cutting etc)

  • What's your "back pocket" wish?
  • Polite company: peace, rest, calm, something like that.

    Honestly: people to leave me the fuck alone and stop asking me to solve their problems and take care of everything. I'm burned out on being responsible for other people.

  • how to make inlays for nail polish for labeling prints

    This seemed neat!

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    I did (not) mean to blow your mind. (Tenacious D x Evangelion)

    I love it so much.

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    Elle, OC of Spookypandagirl

    https://x.com/Spookypandagirl/status/1799160830023319674

    any other communities y'all think this would fit in?

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    Are you joining her for the hunt by RobustaArt

    https://www.instagram.com/p/C77SVU4SJ_E/

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    Chibi Rebecca from Edgerunners by TheArtGun

    https://x.com/TheArtGun/status/1799496188170781042

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    Zelda from Twilight Princess by BeneceaDraws

    https://x.com/BeneceaDraws/status/1799112556797423768

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    finally found time to brew, choc hazelnut porter

    Based on this https://www.morebeer.com/products/chocolate-hazelnut-porter-jamil-zainasheff-grain-kit.html but scaled down to 3 gallons. Came closer to 2 tho. Lost more than expected to boil, I guess.

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    Gundam Calibarn by @yumesan_yume

    https://x.com/yumesan_yume

    https://www.pixiv.net/en/artworks/110210364

    https://x.com/ESPER995/status/1797976527587737835

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    Ogre-class Titan (by Polilla)

    cross-posted from: https://sopuli.xyz/post/13459200

    > Artist: Polilla | twitter | danbooru

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    Yamato (by Kobayashi Makoto)

    cross-posted from: https://sopuli.xyz/post/13448538

    > Artist: Kobayashi Makoto

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    Cyberpunk @lemmy.ml GrayBackgroundMusic @lemm.ee
    [ s y n t h e t i c ] by koyori_n

    cross-posted from: https://lemm.ee/post/33621243

    > https://www.instagram.com/p/C7fYmyIhfD1/

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    Anime armor and swords pics @lemm.ee GrayBackgroundMusic @lemm.ee
    Vhaala, draenei from World of Warcraft, by Actual Sailboat

    https://x.com/actualsailboat/status/1796515136934740186

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    Samus in suit, from Metroid, by Kazumasa Yasukuni

    https://x.com/Kazumasa_yskn/status/1556138301949886466

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